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. 2021 Mar 31;12:630311. doi: 10.3389/fpls.2021.630311

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Copyright © 2021 Cui, Gao, Wang, Lv, Li, Zheng, Wu, Yao, Ding, Li, Zhu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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eIF2α phosphorylation selectively regulates gene transcription under cold stress conditions. (A) The immunoblotting assays of antibodies against phosphorylated eIF2α under normal temperature and cold stress conditions. Western blot results with Actin used as a control. (B) Hierarchical clustering analysis of cold-ATGCN1 genes at transcriptional levels. (C) GO terms analysis of cold-ATGCN1 down-regulated genes at transcriptional levels. (D) GO terms analysis of cold-ATGCN1 up-regulated genes at transcriptional levels. (E) Cold-ATGCN1 down-regulated genes selected for validation by using qRT-PCR. (F) Cold-ATGCN1 up-regulated genes selected for validation by using qRT-PCR. CK, grown in normal temperature conditions. Cold or Cold24, treated by cold stress conditions of 4°C temperature for 24 h treatment. qRT-PCR was repeated three times with similar results attained.