Figure 4.

(A) Kaplan-Meier curves of OS and DFS showing differences between subgroups with and without specific co-mutations of two DDR pathways. The subgroups carrying co-mutations in CPF + FA or FA + HRR pathways had significantly worse OS, and the FA + HRR subgroup also had significantly worse DFS. The results with insignificant correlations were shown in Supplementary Table 3 . (B) the patients carrying mutations in more than one DDR pathway had the worst OS comparing with those with 1 or 0 mutated DDR pathway mutations. The DFS of these three subgroups were also distinguishable, but with less significance. (C) Result of multivariable Cox proportional hazards regression analysis of OS, including the number of mutated DDR pathways, as well as clinical factors previously reported independently associated with CRC prognosis. Carrying more than one mutated DDR pathways maintained significant negative correlation with OS (HR = 9.14, 95% CI: 1.21 – 68.9). The analysis of DFS showed no significance, and result was attached in Supplementary Figure 4 .