TABLE 1.
Extracellular levels of nucleotides on neutrophils and on endothelial cells.
| Extracellular concentration of nucleotides (nmol/L) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| ATP | ADP | AMP | ADO | ||||||
| 3 min | 8 min | 3 min | 8 min | 3 min | 8 min | 3 min | 8 min | ||
| PMN | Vehicle | 59.5 ± 17.5 | 64.1 ± 16.8 | 60.7 ± 17.1 | 55.7 ± 20.3 | 253.9 ± 1.2 | 289.3 ± 34.5 | 38.2 ± 5.5 | 44.5 ± 6.7 |
| ABC (20 µmol/L) | 51.5 ± 7.5 | 50.8 ± 7.6 | 47.9 ± 6.9 | 43.8 ± 0.3 | 224.9 ± 28.4 | 209.5 ± 50.5 | 34.6 ± 0.6 | 32.6 ± 7.8 | |
| HUVEC | Vehicle | 84.5 ± 23.3 | 55.1 ± 12.6 | 32.5 ± 7.9 | 28.9 ± 2.9 | 9.9 ± 0.5 | 10.3 ± 1.3 | 15.5 ± 10.8 | 21.5 ± 4.1 |
| ABC (20 µmol/L) | 66.8 ± 9.4 | 54.55 ± 7.5 | 25.1 ± 7.2 | 25.1 ± 2.8 | 10.4 ± 2.1 | 11.4 ± 0.3 | 19.3 ± 8.7 | 19.2 ± 11.3 | |
PMN and HUVEC were treated with ABC (20 μmol/L) or vehicle, and the supernatant was collected for 3 and 8 min. Concentrations of ATP, ADP, AMP and Adenosine were quantified by HPLC. Results are mean ± SEM, PMN vehicle: n ≥ 4, PMN ABC (20 μmol/L): n ≥ 4, HUVEC vehicle: n ≥ 5, HUVEC ABC (20 μmol/L): n ≥ 5.