TABLE 1.
Quantification of percentage of senescent cells in old (18–32 m), wild‐type, C57Bl/6 mice. DSBs is “Double‐strand breaks”; SA‐β‐gal is “senescence‐associated‐beta‐galactosidase”; EM is “Electron microscopy”; FC is “Flow cytometry”; scRNA‐seq is “single‐cell RNA sequencing”
| Percentage of senescent cells in old (18–32 m), wild‐type, C57Bl/6 mice | |||
|---|---|---|---|
| Article | Senescence marker | Tissue (cell type if specified) | % of positive cells |
| Jurk et al. (2012) | DSBs | Brain (Purkinje neurons) | ~40% |
| Lipid peroxidation | Brain (Purkinje neurons) | ~80% | |
| colorimetric SA‐β‐gal | Brain (Purkinje neurons) | ~60% | |
| DSBs | Brain (cortical neurons) | ~40% | |
| Lipid peroxidation | Brain (cortical neurons) | ~20% | |
| colorimetric SA‐β‐gal | Brain (cortical neurons) | ~80% | |
| Hewitt et al. (2012) | Telomere‐associated DSBs | Small intestine (enterocytes) | 30–40% |
| Telomere‐associated DSBs | Liver (hepatocytes) | 15–25% | |
| Zhu et al. (2015) | colorimetric SA‐β‐gal | Inguinal Fat | ~8% |
| Xu et al. (2015) | colorimetric SA‐β‐gal | Visceral Fat | ~20% |
| Birch et al. (2015) | Telomere‐associated DSBs | Lungs | ~20% |
| Biran et al. (2017) | FC‐based SA‐β‐gal | Inguinal Fat (stromal cells) | ~12% |
| FC‐based SA‐β‐gal | Spleen (stromal cells) | ~4% | |
| FACS‐based SA‐β‐gal | Small intestine (stromal cells) | ~3% | |
| Baker et al. (2016) | EM‐based SA‐β‐gal | Visceral Fat | ~2% |
| EM‐based SA‐β‐gal | Kidney | ~2% | |
| EM‐based SA‐β‐gal | Heart | ~10% | |
| FC‐based p16 (GFP) | Inguinal Fat | ~5% | |
| Ogrodnik et al. (2017) | DSBs | Liver (hepatocytes) | ~15% |
| Telomere‐associated DSBs | Liver (hepatocytes) | ~15% | |
| Anderson et al. (2019) | Telomere‐associated DSBs | Heart (cardiomyocytes) | 50–70% |
| colorimetric SA‐β‐gal | Heart (cardiomyocytes) | ~4% | |
| Lipid peroxidation | Heart (cardiomyocytes) | ~70% | |
| DNA oxidation | Heart (cardiomyocytes) | ~20% | |
| Liu, Souroullas, et al. (2019)) | FC‐based p16 (tdTom) | Pancreas | ~3% |
| FC‐based p16 (tdTom) | Inguinal Fat (progenitor cells) | ~6% | |
| FC‐based p16 (tdTom) | Cartilage | ~6% | |
| Iske et al. (2020) | p16/p21 (antibody) | Skin | ~8% |
| p16/p21 (antibody) | Heart | 9–10% | |
| colorimetric SA‐β‐gal | Kidney | ~10% | |
| Tabula Muris (2020) | scRNA‐seq‐based p16 | Multiple tissues | 2–3% a |
a
Converted from fraction.