FIGURE 1.

Experimental workflow for combined metabolomics and lipidomics of the starvation response in C. elegans. Wild‐type C. elegans and the hlh‐30 mutant were included in the study. Starvation was induced by transferring animals to plates containing no bacteria. Worms were starved for 1, 2, 3, 4, 6, and 16 h of starvation, prior to harvesting and extraction of metabolites and lipids. The upper aqueous phase was analyzed using LC–MS‐based metabolomics, and the lower organic phase was analyzed using LC–MS‐based lipidomics