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. 2021 Mar 6;20(4):e13322. doi: 10.1111/acel.13322

FIGURE 5.

FIGURE 5

Effect of diet and aging on skeletal muscle proteostasis. (a) Fractional synthesis rate (FSR) of the myofibrillar, cytoplasmic, and mitochondrial fractions from the GTN of 16‐month‐old mice on a control (CON) or ketogenic diet (KD). Values are expressed as means ± SEM. (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001) comparing CON and KD (b, c) Protein levels and representative western blot images of proteins associated with protein synthesis from the GTN of 16‐ and 26‐month‐old mice on a CON or KD. (d) ATP‐independent (20S) and ATP‐dependent (26S) proteasomal subunit (β1, β2, and β5) activities from the quadricep of 16‐ and 26‐month‐old mice on a CON or KD. (e, f) Protein levels and representative western blot images of proteins associated with autophagy from the GTN of 16‐ and 26‐month‐old mice on a CON or KD. For complete western blot images, refer to Appendix S1 (Figure S4). Values are expressed as means ± SEM. n = 6 (16‐month CON), n = 7 (16‐month KD and 26‐month CON), and n = 9 (26‐month KD). (#) main effect of age. ($) main effect of diet. (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001) comparing CON and KD at 16 and 26 months. (a) p < 0.05 comparing CON at 16 and 26 months. (b) p < 0.05 comparing KD at 16 and 26 months