FIGURE 1.

GHR/GHBP knockout mice with CRISPR technology have altered body composition. The alignment of exon 4 (gray rectangle) reference sequence with electropherograms from wild‐type and mutant (28208) alleles (a). The translated sequence of single letter amino acids (maroon rectangle) shows the five amino acids inferred to be deleted in the mutant allele (orange rectangle) and position of a missense mutation at residue E44 (blue rectangle) in a young human patient. The CRISPR target site is indicated by the blue bar, with a red arrowhead representing the PAM (NGG). The red rectangle with dotted lines in the bottom electropherogram indicates the 15 bp deleted sequence. Body weights of WT and D‐KO mice from weaning to adulthood (b). Body composition parameters were measured by DXA. Body composition parameters were plotted on the y‐axis, and body weights are plotted on the x‐axis (c‐f). Relationship between body weight and BMD in WT and D‐KO mice (c). Relationship between body weight and BMC in WT and GHRH^‐/‐ mice (d). Relationship between body weight and lean mass in WT and D‐KO mice (e). Relationship between body weight and fat mass in WT and GHRH^‐/‐ mice (f). WT n = 12, D‐KO n = 11. The WT and D‐KO groups were statistically analyzed with ANCOVA method, which was used to calculate p values, shown on each panel