Figure 1. Design, generation, characterization, and expression analysis of Prostate Cancer Antigens (PCaA) SEV constructs.

(A) Schematic representation of the PCaA-SEV construct generated. (B) Western blot analysis of SEV of STEAP-1, PAP, PARM1, PCTA, PSCA and PSP94. Human 293T cells were transfected with 2μg of each DNA vaccines or pMV101 and the cell lysates were collected after 48 h. 25μg of each lysate was then separated using polyacrylamide gel electrophoresis and subsequently transblotted; followed by the incubation with respective primary and then secondary antibodies. Cell lysates transfected with different PCaA- SEV revealed the correct molecular sized bands corresponding to the expression of respective proteins; however, no bands were present in pMV101 lanes. β-actin was used as loading control in all the cases.