Figure 3. PCaA-SEV induces both CD4⁺ and CD8⁺ T cell responses in mice.

Splenocytes from PCaA-SEV immunized mice as shown in the previous experiments were also evaluated by polychromatic flow cytometry to measure (A) CD4⁺ and (B) CD8⁺ T cells producing different cytokines. Splenocytes obtained from mice, after three immunizations of PCaA-SEV or pMV101, were stimulated with respective PCaA target peptides ex vivo and then stained with different fluorophore-tagged antibodies as shown, for determining the production of cytokines by both CD4⁺ and CD8⁺ T cells. Graphs indicate the total percentage of IFN-γ⁺, TNF-α⁺, and IL-2⁺ T cells (mean ± SEM). PCaA-SEV resulted in higher frequency of CD4⁺ as well as CD8⁺ cells secreting intracellular cytokines upon ex vivo stimulation with antigen specific peptides.