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. 2021 Mar 22;12:51–64. doi: 10.18632/genesandcancer.214

Figure 4. PCaA-SEV induces humoral immune response.

Figure 4

(A) ELISA reactive antibodies following the third dose of immunization with PCaA-SEV (day 35). Sera were diluted as shown and vaccine-specific IgG reacting with each antigen was determined through ELISA. Mean optical density and SEM for each group/ dilution against each antigen is indicated. (B) Indirect immunofluorescence analysis of prostate antigen expression in HepG2 cells expressing different PCaA-SEV to confirm whether antibodies induced by the experimental prostate antigen could recognize vaccine-transfected cells. 48hrs post transfection of HepG2 cells, incubated with pooled day 35 sera (1:100) from mice immunized with different PCaA-SEV (50ug/immunization); Alexa Fluor 488-tagged anti-mouse IgG secondary antibody (green) and DAPI (blue) were used in this assay.