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SCID/Beige mice were injected, via tail vein, with 5 × 105 GFP-labeled MDA-IBC3 cells and irradiated with a single 4-Gy fraction (opposing lateral fields as indicated by the red circle) at the indicated times; mice were sacrificed at 4 weeks or at 8 weeks after tumor-cell injection and their brain was examined for the presence of metastases.
