FIGURE 5.

Placenta mesenchymal stromal cells (P‐MSCs) induce more IL‐10‐producing Breg subsets than bone marrow (BM)‐MSCs in vivo. A, Flow cytometric gating strategy for identifying IL‐10 producing transitional 2‐marginal zone precursor (T2‐MZP) subset (CD19⁺ CD23⁺ IgM^hi CD21^hi IL‐10⁺). B, tSNE plots of IL‐10⁺ T2‐MZP subset (n = 5 in each group) identified in experimental conditions as denoted, with overlay of manually gated IL‐10⁺ cells (navy blue) on T2‐MZP (light blue); and C, pooled data. D, Flow cytometric gating strategy for identifying IL‐10 producing MZ B subset (CD19⁺ CD23⁻ IgM^hi CD21^hi IL‐10⁺). E, tSNE plots of IL‐10⁺ MZ B subset (n = 5 in each group) identified in experimental conditions as denoted, with overlay of manually gated IL‐10⁺ cells (navy blue) on MZ B cells (orange); and F, pooled data. tSNE plots (B,E) were generated by concatenation of individual samples. tSNE map of all samples (left‐most map) were generated from combining sample files of PBS control, lipopolysaccharide (LPS)‐challenged, BM‐MSC‐, and P‐MSC‐adoptive transfers. tSNE analysis was run on 6000 live CD19⁺ single cells per sample using five markers: CD19, CD23, IgM, CD21, and IL‐10. All samples = 120 000 events; individual PBS control and LPS‐challenged samples = 30 000 events (n = 5); individual MSCs adoptive transfer samples = 30 000 events (n = 5). Data are shown as mean ± SD. *P < .05; **P < .01; ***P < .001