FIGURE 5.

Inhibition of ferroptosis by Fer‐1 attenuates cell death induced by curcumin in A549 and H1299 cells. (a) The viability of A549 and H1299 cells with 30 μmol/L curcumin and 10 μmol/L Fer‐1 treatment for 24 hours. (b) Representative cell morphological changes in each group of cells (scale bar = 200 μm). (c) Effects of Fer‐1 on the curcumin‐induced accumulation of lipid ROS in A549 and H1299 cells. The level of ROS was detected by fluorescent probe DCFH‐DA. (d)–(f) Effects of curcumin and Fer‐1 on MDA, SOD, GSH, and iron content in A549 and H1299 cells. (g), (h) Effects of curcumin and Fer‐1 on the protein level of ACSL4, SLC7A11, and GPX4. All experiments were done more than three times independently, and all results are expressed as the mean ± SD. *P < 0.05, **P < 0.002, ***P < 0.0002, ****P < 0.0001 versus curcumin group