FIGURE 7.

Curcumin‐induced cellular ferroptosis was alleviated by CQ. (a) Cell viability was measured after 30 μmol/L curcumin and 20 μmol/L CQ treatment for 24 hours. (b) Representative cell morphological changes in each group of cells (scale bar = 200 μm). (c) Effects of curcumin and CQ on autolysosome and ultrastructural features of mitochondrion in A549 and H1299 cells (scale bar = 10 μm). (d) The effect of CQ on protein levels of P62, Beclin1, LC3, ACSL4, SLC7A11, and GPX4 in curcumin‐treated A549 and H1299 cells. (e) Semi‐quantitative analysis of immunoblotting results. (f)–(h) Effects of curcumin and CQ on MDA, SOD, GSH, and iron content in A549 and H1299 cells. All results are expressed as the mean ± SD. *P < 0.05, **P < 0.002, ***P < 0.0002, ****P < 0.0001 versus curcumin group