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. 2021 Apr 14;16(4):e0249954. doi: 10.1371/journal.pone.0249954

Fig 3. HSP gene expression 3 h after exposure of NSCs to heat shock.

Fig 3

NSCs cultured for 24 h (day 0; white) or 96 h (day 3; black) under proliferation conditions were exposed to heat shock (HS) at 43°C or 44°C and were then allowed to recover for 3 h. The expression of (A) Hsp70, (B) Hsp40, (C) Hsp90, and (D) Hsp27 was assayed by real-time RT-PCR and normalized by that of RPS29. Results are presented as means ± SEM (n = 4) (*P <0.05, **P <0.01 between 24 h and 96 h, ††P <0.01, †††P <0.001 between 37°C and 43°C or 44°C).