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. 2021 Apr 14;16(4):e0249954. doi: 10.1371/journal.pone.0249954

Fig 4. Expression of apoptosis-related genes 3 h after exposure of NSCs to heat shock.

Fig 4

NSCs cultured for 24 h (day 0; white) or 96 h (day 3; black) under proliferation conditions were exposed to heat shock (HS) at 43°C or 44°C and were then allowed to recover for 3 h. The expression of (A) cytochrome c, (B) Apaf-1, (C) caspase-9, (D) caspase-3 and (E) caspase-7 was assayed by real-time RT-PCR and normalized by that of RPS29. Results are presented as means ± SEM (n = 4). (**P <0.01 between 24 h and 96 h, †P <0.05, ††P <0.01, †††P <0.001 between 37°C and 43°C or 44°C).