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. 2021 Apr 14;16(4):e0249954. doi: 10.1371/journal.pone.0249954

Fig 5. Caspase activities of NSCs exposed to heat shock.

Fig 5

(A) Cell viability was measured 3 h after exposure to heat shock (HS) using CellTiter Glo reagent (Promega), with results expressed as mean ± SEM (n = 8) ratios to the 37°C sample. (**P <0.01 for comparisons between cells exposed to HS after culture for 24 h (day 0; white) and 96 h (day 3; black); †††P <0.01 for comparisons between 37°C and 43°C or 44°C). (B) Caspase activity was measured 3 h after exposure to heat shock (HS) using the caspase-Glo 3/7 reagent (Promega) and normalized to cell viability measured using CellTiter Glo reagent (Promega), with results expressed as mean ± SEM (n = 8) ratios to the 37°C sample. The values are presented as the in the graph. (**P <0.01 for comparisons between cells exposed to HS after culture for 24 h (day 0; white) and 96 h (day 3; black); †††P <0.01 for comparisons between 37°C and 43°C or 44°C).