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. Author manuscript; available in PMC: 2021 Apr 14.
Published in final edited form as: Neuron. 2019 Feb 28;102(2):435–449.e6. doi: 10.1016/j.neuron.2019.02.006

Figure 6. Developmental Microglia Phagocytosis Programs Juvenile Play Behavior, Astrocyte Density, and Neuronal Activation in the MePD.

Figure 6.

(A) Schematic showing the treatment paradigm and timeline for (B)–(D).

(B) Nissl-stained coronal section of the P26 brain (top). Dashed box indicates location of the MePD. Representative images of female (bottom left) and male (bottom right) sections immunolabeled for zif268 and DAPI are shown. Dashed lines indicate boundaries of the MePD. Scale bars represent 200 μm.

(C) Quantification of the density of zif268+ cells in the MePD. ANOVA F(2, 45) = 7.877; p = 0.00117. Dunnett’s post hoc comparisons to male vehicle are shown. n = 16 rats per group.

(D) Quantification of the density of GFAP+ cells in the MePD. ANOVA F(2, 45) = 7.266; p = 0.00184. Dunnett’s post hoc comparisons to male vehicle are shown. n = 16 rats per group.

Bars represent the mean ± SEM. Open circles represent individual data points for each animal. **p < 0.01 and ***p < 0.001.

See also Figure S6.