Figure 3.

Flow Cytometry Analysis of Myricetin-Induced T47-D Cells. (A) The dot plot diagrams represent typical apoptotic and necrotic cell populations detected by Annexin V-FITC and PI staining. The lower left quadrants of the panels show viable intact cells, which were negative for Annexin V-FITC binding and excluded PI staining (FITC-/PI-); the upper right quadrants show late apoptotic cells, which were positive for Annexin V-FITC binding and PI uptake (FITC+/PI+). The lower right quadrants represent early apoptotic cells, positive for Annexin V-FITC and negative for PI (FITC+/PI-). (B) Bar graph shows average percentage of apoptotic cells. The values are presented as means ± SD. *p<0.01