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. 2021 Apr 14;16(4):e0243333. doi: 10.1371/journal.pone.0243333

Fig 2. Optimization of the assay.

Fig 2

(A) Total RNA from 18 clinical samples with a wide range of SARS-CoV-2 infection were subjected to either a Two-step reaction (red circles; consecutive Reverse Transcription and Pre-amplification) or One-step reaction (blue circles; combined Reverse Transcription and Pre-amplification). Quantitative PCR reactions were performed on the Biomark-HD using cellular (RNP) and viral (N, E, ORF1ab) primers/probe sets. (B) Total RNA from 18 clinical samples with a wide range of SARS-CoV-2 infection were extracted with either the miRNeasy Advanced Serum Plasma Kit (red circles) or the Virus QIAamp Viral RNA kit (blue circles). RNAs were processed using the One step reaction. Quantitative PCR reactions were performed on the Biomark-HD using cellular (RNP) and viral (N, E, ORF1ab) primers/probe sets. The Cq presented are representative of two independent experiments performed in quadruplicate.