Fig 6. Roles of TLR2 and NLRP3 receptors in GEVs triggered inflammatory response.
Murine peritoneal macrophages were pre-treated with 100 μM C29, 100 μM Glibenclimide, or 25 μM CA-074 Me for 1 h and then added 25 μg/mL GEVs or 1.5 × 106 parasites/mL G. duodenalis for 24 h. PBS-treated was used as negative control (NC) and GEVs or G. duodenalis-treated alone was used as positive control (PC). (A) Supernatants were collected and the protein expression levels of inflammatory cytokines, including IL-6, IL-1β, and TNF-α were measured using ELISA assays. (B) Protein expression level of inflammatory cytokine IL-1β in the culturing supernatants was detected using western blotting assay and densitometric percentage of IL-1β and actin in GEVs and G. duodenalis were calculated. Results are representative of three independent experiments with three technical replicates and data are mean±SEM. *p < 0.05, **p < 0.01 or ***p <0.001 vs. PC of GEVs group or G. duodenalis treated group.