Table 2. Primers used for qRT-PCR analysis of TLRs.
| Gene | Genbank number | Primer sequence (5’to 3’) | Product size (bp) | Primer length (nt) | Cross intron length (nt) | Primer site |
|---|---|---|---|---|---|---|
| TLR1 | NC_000071 | AGTCAGCCTCAAGCATTTGG | 115 | 20 | 0 | 6716. . .6735 |
| TACCCGAGAACCGCTCAAC | 19 | 6830…6812 | ||||
| TLR2 | NC_000069 | CGCTCCAGGTCTTTCACCTC | 101 | 20 | 0 | 3165…3184 |
| AGGTCACCATGGCCAATGTA | 20 | 3265…3246 | ||||
| TLR3 | NC_000074 | CGCAGTTCAGCAAGCTATTG | 103 | 20 | 0 | 13950…13969 |
| TCTTCGCAAACAGAGTGCAT | 20 | 14052…14033 | ||||
| TLR4 | NC_000070 | ACTGTTCTTCTCCTGCCTGACA | 99 | 22 | 5974 | 323…344 |
| GGACTTTGCTGAGTTTCTGATCC | 23 | 6395…6373 | ||||
| TLR5 | NC_000067 | GGATGCTGAGTTCCCCCAC | 134 | 19 | 700 | 17686…17704 |
| AAAGGCTATCCTGCCGTCTG | 20 | 18519…18500 | ||||
| TLR6 | NC_000071 | TCCGACAACTGGATCTGCTC | 101 | 20 | 0 | 15886…15905 |
| AAGACTTTCTGTTTCCCCGC | 20 | 15986…15967 | ||||
| TLR7 | NC_000086 | CCGTTGAGAGAGTTGCGGTA | 187 | 20 | 0 | 23707…23726 |
| TGAGTTTGTCCAGAAGCCGTA | 21 | 23893…23873 | ||||
| TLR8 | NC_000086 | CTGACGTGCTTTTGTCTGCTG | 101 | 21 | 0 | 18508…18528 |
| AGGGAGTTGTGCCTTATCTCGT | 22 | 18608…18587 | ||||
| TLR9 | NC_000075 | CTGCCCAAACTCCACACTCT | 100 | 20 | 0 | 2082. . .2101 |
| ACAAGTCCACAAAGCGAAGG | 20 | 2181…2162 | ||||
| TLR11 | NC_000080 | TTGGGATTGGAAATGACAGG | 173 | 20 | 0 | 5104…5123 |
| CAACAGCAGGAGATGAGTGG | 20 | 5276…5257 | ||||
| TLR12 | NC_000070 | TAACTGGGTGGAGCACTTCC | 143 | 20 | 0 | 214…233 |
| CAAGGTCTGTGTCAGGTTGC | 20 | 356…337 | ||||
| TLR13 | NC_000086 | AAAGACACGGGATTCAGGTTG | 102 | 21 | 0 | 15192…15212 |
| GGTGGTCCAGGAATACAGAGG | 21 | 15293…15273 |