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. 2020 Apr 19;11(2):351–360. doi: 10.34172/apb.2021.033

Figure 5.

Figure 5

(A) Hesperetin modulates the cell cycle in monolayers and mammospheres. Results are shown as mean±SD (n=3). (B) Hesperetin induces apoptosis in monolayer and mammospheres. Results are shown as mean±SD (n=3). (C). Modulation of mRNA expression of p21, p53, MMP9, NOTCH, HES1, PPARG and CMYC in MCF-7 monolayers and mammospheres. mRNA expression of stemness regulatory genes vimentin, E-cadherin, ß-catenin, ALDH1, and MMP9 upon hesperetin treatment in (D) MCF-7 monolayer cells and (E) MCF-7 mammospheres. mRNA expression of apoptosis and cell-cycle regulatory genes caspase-7, Bax, BCL2, survivin, cyclinD, p21, and PCNA upon hesperetin treatment in (F) MCF-7 monolayer cells and (G) MCF-7 mammospheres. mRNA expression of p53, NOTCH1, HES1, PPARG, and CMYC upon hesperetin treatment in (H) MCF-7 monolayer cells and (I) MCF-7 mammospheres. Gene-expression analysis was conducted using q-RT PCR with selected primers as described in the methods section. GAPDH was used as a housekeeping gene. Results are shown as mean±SD (n=3). Statistical analyses were conducted using Student's t test. ***P < 00.001, **** P <0.0001.