Figure 2. RNA polymerase 3 transcription defects induced by lack of Sen1 trigger the accumulation of condensin.

(A) Cells were synchronized in metaphase and the association of condensin (Cnd2-GFP) or the heterologous LacI (lacI-GFP) at the indicated loci was investigated by ChIP-qPCR in the presence and in the absence of Sen1 (mean ± std of four biological replicates; P-values determined by the test of Wilcoxon Mann-Whitney are indicated above the graph). (B) The association of the TFIIIC component Sfc6 at the indicated loci was investigated by ChIP-qPCR in cells synchronized in metaphase (mean ± std of five biological replicates). (C, D) Distribution of condensin (cnd2-GFP, top) and RNA polymerase 3 (rpc37-flag, bottom) around SPCTRNATHR.10 (C) and SPCTRNAARG.10 (D) in mitotic cells, in the presence or not of super-terminator sequences (thr10-20T and arg10-23T, respectively) which correct the transcription termination defects in the absence of Sen1 (Rivosecchi et al, 2019) (compare the yellow and red curves). (C, D) Results are presented as (mean ± std) of three (C) or four (D) biological replicates.

Source data are available for this figure.