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Myogenic Immunohistochemical Markers Antibody Source Clone Dilution Pretreatment
| Antibody | Source | Clone | Dilution | Pretreatment |
|---|---|---|---|---|
| SMA | Leica Biosystems | ASM-1 | RTU | None |
| Desmin | Leica Biosystems | DE-R-11 | RTU | ER2 for 20 min |
| Caldesmon | Bio SB Cancer Diagnostics | CALP | RTU | ER2 for 10 min |
| Calponin | Agilent Dako | 26a11 | 1:1600 | ER2 for 10 min |
| Myogenin | Leica Biosystems | LO26 | RTU | ER2 for 40 min |
ER2 indicates Leica Bond Epitope Retrieval Solution 2 heat-induced epitope retrieval; RTU, ready to use, no dilution.
