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. 2021 Apr 5;2021:6662612. doi: 10.1155/2021/6662612

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Copyright © 2021 Chunting Lu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

miR-720 regulates insulin secretion by targeting Rab35 in MIN6 cells. (a) miR-720 mimic could downregulate the Rab35 mRNA expression. (b) miR-720 mimic could downregulate the Rab35 protein expression. (c) miR-720 inhibitor could upregulate the Rab35 mRNA expression. (d) miR-720 inhibitor could upregulate the Rab35 protein expression. (e) Relationship between miR-20a and its target Rab35 is based on dual luciferase reporter gene assays. (f) Western blot showed that the constructed vector pcdna3.0-rab35 had a high expression level. (g) Compared with the control group transfected with empty pcdna3.0-vector, the Rab35 overexpression promoted insulin secretion. ^∗P < 0.05.