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. 2021 Mar 31;11:657094. doi: 10.3389/fonc.2021.657094

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Copyright © 2021 Li, Li, Wang, Liang, Luo, Han, Li, Chen, Zhang, Liu, Wang, Chen, Wang, Zhao and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LINC01977 overexpression promoted breast cancer cell proliferation, metastasis, and chemoresistance to DOX. (A) The efficiency of LINC01977 expression was evaluated by real-time PCR in MDA-MB-231 and MCF-7 cells. (B) MTT assay. (C) Colony formation assay. (D) EDU assay. (E) IC50. (F) Cytotoxicity assay. (G) Apoptosis assays. As shown in right panel, total apoptosis cells were quantified. (H) Transwell assay. Left panel: Cell migration assay. Right panel: Cell invasion assay. PCDH: empty PCDH vector using as negative control. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; Student’s t-test).