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. 2021 Mar 31;11:657094. doi: 10.3389/fonc.2021.657094

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Copyright © 2021 Li, Li, Wang, Liang, Luo, Han, Li, Chen, Zhang, Liu, Wang, Chen, Wang, Zhao and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LINC01977 served as a molecular sponge via binding to miR-212-3p. (A, B) The subcellular location of LINC01977. β-actin: positive control for cytoplasm; U6: positive control for nuclear. (C) Left panel: Real-time PCR analysis of LINC01977 and miR-212-3p enriched by Ago2 proteins in MCF-7 cells. Right panel: Western blot was used to confirm the specific immunoprecipitation of Ago2. (D, E) Real-time PCR confirmed the interaction between LINC01977 and miR-212-3p in MDA-MB-231 and MCF-7 cells. (F) A schematic of wild-type LINC01977 luciferase reporter vectors. (G) Dual-luciferase reporter assay. NC: negative control of miR-212-3p mimics. (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001; Student’s t-test).