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. 2021 Apr 14;11:8183. doi: 10.1038/s41598-021-87791-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Keratocytes’ viability was measured after different concentrations (1 μM to 1000 mM) of sodium nitrite exposure for 24 (A), 48 (B) and 72 h (C). Sodium nitrite did not harm cultured keratocyte viability up to 10 mM concentration for 72 h. However, high concentrations (100 mM or 1000 mM) of sodium nitrite decreased keratocyte viability. *p < 0.05, ***p < 0.001.