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. 2021 Apr 14;11:8183. doi: 10.1038/s41598-021-87791-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Myofibroblast marker expression by TGFβ1 stimulation in keratocytes. (A) Western blot analysis after 24 h TGFβ1 stimulation. TGFβ1 increased αSMA and N-cadherin expression from keratocytes. Smad3 phosphorylation is also increased with TGFβ1 stimulation. (B) Immunocytochemical staining revealed enhanced αSMA positivity (green: αSMA, red: F-actin, blue: DAPI, white bar: 50 μm) with higher concentrations of TGFβ1 stimulation for 72 h. Note that only the bands at the adequate molecular weights were shown here. Full length gel and blots are included in the Supplementary Information. *p < 0.05, **p < 0.01.