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. 2021 Apr 14;11:8183. doi: 10.1038/s41598-021-87791-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of NO on TGFβ1-stimulated keratocytes. αSMA and N-cadherin expression in TGFβ1-stimulated keratocytes significantly decreased after 1000 μM sodium nitrite treatment for 24 h. However, lower concentration (100 μM) of sodium nitite showed little effect. Smad3 phosphorylation was also decreased after 1000 μM sodium nitrite treatment for 24 h. Addition of 2-4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO, 10 μM) in the culture medium reversed the effect of NO. Statistical analysis was performed by setting the expression level in TGFβ1-stimulated keratocytes with no NO treatment as the baseline. Note that only the bands at the adequate molecular weights were shown here. Full length gel and blots are included in the supplementary information. *p < 0.05, **p < 0.01, ***p < 0.01.