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. 2021 Apr 14;11:8183. doi: 10.1038/s41598-021-87791-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo effect of NO on corneal opacity development after alkali burn. (A) Representative photographs of the alkali burned murine cornea at day 0 and 14 days after injury. Green stained areas by fluorescein at day 0 represent corneal epithelial defect induced by alkali burn. Corneal opacity of NO treated corneas (100 μM and 1000 μM) was significantly better than PBS control observed at 2 weeks. (B) The difference of corneal opacity grades between treatments and control (PBS) was significant. However, the difference between 100 and 1000 μM of sodium nitrite treatments was not significant. (C) Histologic examination revealed significantly increased corneal thickness and stromal cellularity (yellow arrows) in PBS treated cornea compared to NO treated cornea. *p < 0.05, scale bar: 100 μm.