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. 2021 Apr 14;11:8133. doi: 10.1038/s41598-021-86980-y

Figure 1.

Figure 1

Quenching reagent effectiveness in small intestine samples. (A) The quenching reagent stock solution (pH 8.5, 50 mM Tris, 150 mM NaCl, 10 mM EDTA, 1.5% SDS, 8 M urea) with/without Ag+ was tested on chicory FOS/inulin and GOS breakdown by one human small intestine sample in vitro in a 24 h incubation. The tested ratios of quenching reagent:fermentation sample were 1:2, 1:3, 1:4 v/v. Fermentation samples were centrifuged and the supernatant was diluted in water, so the maximum fibre concentration became 0.5 mg/mL, and analyzed by HPAEC-PAD. (B, C) The percentage of GOS DP > 2 (B) or FOS/inulin DP > 2 (C) after 48 h in vitro fermentation by five human small intestine samples in the presence of quenching reagent (pH 8.5, 175 mM Tris, 525 mM NaCl, 35 mM EDTA, 12% SDS, and 8 M urea) or control, 1:5 v/v. Each line represents one individual. Breakdown was expressed as % remaining carbohydrates after incubation versus the initial carbohydrates present at 0 h. GOS galacto-oligosaccharides, FOS fructo-oligosaccharides.