Skip to main content
. 2021 Apr 14;11:8133. doi: 10.1038/s41598-021-86980-y

Figure 2.

Figure 2

Quenching reagent interference in SCFA, oligosaccharides and bacterial 16S rRNA copy number analysis. (A) Calibration curves made of acetate concentrations in PBS in the presence of quenching reagent or its major components. (B) Calibration curve of acetate after precipitation of SDS with KCl in different KCl/SDS molar ratios. HPAEC chromatograms of GOS (C, E) and FOS (D, F) prepared in water. The black lines represent the mixture of fibre in water, and the red lines represent the fibre in water in the presence of quenching reagent (C, D) or in the presence of quenching reagent after precipitation of SDS with KCl (E, F). The numbers in the chromatograms represent the degree of polymerization, 1 = monomers, 2 = dimers, ≥ 3 = oligomers, and * are components present in the quenching reagent. (G) The % 16S rRNA bacteria copy number present in the supernatant or in the pellet versus total from two faecal fermentation samples were calculated in the presence of quenching reagent or PBS at 0 and 48 h. The total is the 16S rRNA copy number in pellet plus supernatant.