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. 2021 Apr 14;11:8133. doi: 10.1038/s41598-021-86980-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Quenching of the 16S rRNA bacteria copy numbers and microbiota composition in human faecal samples. (A) The total bacteria 16S rRNA copy numbers in the faecal fermentation samples, that were mixed and diluted in SIEM medium with fibres, in the presence of quenching reagent or PBS at 0 and 48 h. The same colour represents faeces from the same individual. (B) The relative microbiota composition at 0 h and 48 h after addition of PBS or quenching reagent ‘Q’ in conditions with added GOS or chicory FOS for two faecal donors. The top 25 genera present in the dataset are shown. Pearson correlation coefficients were calculated on the relative abundances at genus level, and shown in the graph as correlation number. Q quenching reagent, FOS fructo-oligosaccharides, GOS galacto-oligosaccharides.