Table 1.

Toxicology information about the components in the quenching reagent.

Chemical name	CAS registration number	Concentration	Amount mg/50 µl	Toxicology information
Trisaminomethane, thrometamine (Tris)	77-86-1	175 mM	1.1	Oral (non-human) NOAEL = 4000 mg/kg
Sodium chloride (NaCl)	7647-14-5	530 mM	1.5	GRAS component
Ethylenediaminetetraacetic acid (EDTA)	60-00-4	35 mM	0.7	EFSA, no safety concern (humans) = 1.9 mg/kg/day
Sodium dodecylsulfate, sodium laurylsulfate (SDS)	151-21-3	12%	6	Oral (non-human) NOAEL = 100 mg/kg/day
Urea	57-13-6	8 M	24	GRAS component

The concentrations of all components and amounts of the components present in 50 µL of the quenching reagent, the CAS registration numbers, and information about toxicology is presented.

CAS chemical abstracts service, GRAS generally recognized as safe, NOAEL no observed adverse effect level, EFSA European Food Safety Authority.