Skip to main content
. 2021 Apr 14;4:370. doi: 10.1038/s42003-021-01897-6

Fig. 6. PTC596 treatment of the KRAS mutant A549 and SKLU1 cell lines affects their cell cycle progression.

Fig. 6

Western blot analyses of human a A549 and b SKLU1 cell lines treated for 24, 48, and 72 hours with DMSO vehicle as control, and PTC596 or PTC028. Protein lysates were immunoblotted with an anti-BMI-1 antibody. Loading was assessed with an anti-β-actin antibody. The expected size is shown in kDa. The slower migrating hyperphosphorylated BMI-1 band is indicated by the arrowhead. cf Cell cycle analysis of the A549 cd and SKLU1 ef cell lines after treatment for 24 hours with DMSO (red), PTC596 (blue), or PTC028 (black). The bar charts (d and f) represent the distribution of cells in G0-G1, S, and G2-M phases. P values are indicated. Error bars represent standard deviation (SD). Percentages of cells in each cell cycle phase are indicated. g Fluorescence nuclear imaging of DNA and H2AK119ub in A549 cell line by confocal microscopy. Upper panels show DMSO-treated cells; lower panels show cells treated with PTC596 for 24 hours. Left panels show DNA staining by Hoechst 33342 (blue acquisition chanel). Right panels show H2AK119ub staining by immunofluorescence (scale bar 10 μm). h Dot plots of H2AK119ub average nuclear fluorescence for DMSO− (red) and PTC596-treated (blue) A549 cells. Error bars represent SD of the average values. Data are expressed in fluorescence counts which are proportional to H2AK119ub concentration. P value is indicated.