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. 2021 Apr 14;11:8163. doi: 10.1038/s41598-021-87097-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Circular dichroism spectra and EMSA corresponding to the DNA and RNA duplexes comprising of CCG repeats. (A) pH and (B) salt-dependent CD spectra showing the preference for duplex conformation by DC6 that contain 6 C…C mismatches. Preference for (C,D) i-motif like conformations by RC6 and RC5, and (E) A-form conformation by RC1. The figures (A–E) were plotted by using MATLAB 7.11.0 software (www.mathworks.com). Gel picture corresponding to (F) DC6 and DC5 (lanes: 4, 5) and (G) RC5 (lane: 1), RC6 (lane: 2), and RC1 (lane: 3) sequences at pH 5 (left), pH 7 (middle), and pH 9 (right). The EMSA (F,G) samples were analyzed by 10% native PAGE and stained with Stains All dye. The unprocessed gel images (F,G) are incorporated in Supplementary Fig. S13D,E.