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. 2021 Apr 14;11:220. doi: 10.1038/s41398-021-01337-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Adult male Long-Evans rats were intermittently exposed to either ethanol vapor (CIE) or air (Air) for 8 weeks. The LHb tissue was harvested at 24 h after vapor off. The mRNA levels of genes associated with endocannabinoid signaling including Cnr1, Napepld, Dagla, Daglb, Faah, Mgll, as well as genes related to excitatory and inhibitory neurotransmission, like aCaMKII, bCaMKII, Gad67, and Gad65 were measured in CIE (n = 11) and air control (Air, n = 6) rats. The protein levels of CB1Rs, MAGL, FAAH, and ABHD6 in the LHb were also measured. Panels (a) and (b) display quantitative mRNA levels and protein expression in these two groups. The Western blot and RT-qPCR tests were repeated three times. All data are expressed as mean ± SEM. *p < 0.05, ***, p < 0.001, CIE vs. Air group revealed by an unpaired t test.