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. 2021 Apr 14;11:8170. doi: 10.1038/s41598-021-87571-7

Figure 2.

Figure 2

Spontaneous cell sheet contraction contributes a 3D tissue-like structure. Cross-sectional visualization of (a) 2D monolayer and (b) 3D cell sheet tissue structure with H&E stain. Quantified comparison of 2D monolayer and 3D cell sheet (c) diameter, (d) thickness, and (e) volume. Scale bars = 200 μm. Values are means ± SE (n = 10 (diameter), n = 3 (thickness): *p < 0.05, ***p < 0.001).