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. 2021 Apr 14;11:8177. doi: 10.1038/s41598-021-87759-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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SIRT1 promoted mitochondrial biogenesis and respiration in 3T3-L1 adipocytes. (a) Representative confocal microscopy images of ShScrambled or SIRT1-depleted (ShSIRT1) adipocytes labelled with 250 nM MitoTracker Green at Day 6 post-differentiation. Scale bar 100 µm. (b) Quantification of MitoTracker Green staining intensity in ShScrambled or SIRT1-depleted (ShSIRT1) adipocytes (normalized to cell number) (n = 3). (c) Quantification of changes in the expression of PGC-1α in ShScrambled or SIRT1-depleted (ShSIRT1) (pre)adipocytes at specific time-points during adipogenesis (n = 4). (d) Evaluation of mitochondrial respiration parameters in ShScrambled or SIRT1-depleted (ShSIRT1) adipocytes using Seahorse technology at Day 6 post-differentiation (n = 12 biological replicates). Statistical analysis of the dataset was performed using a Student’s t test. For statistical analysis of data shown in (c), pair-wise comparisons were made between ShScrambled and ShSIRT1 groups for each individual time-point using the Student’s t test (*P < 0.05; ****P < 0.0001; n.s., not significant).