Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 14;11:8173. doi: 10.1038/s41598-021-87696-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Ginkgetin treatment does not alter total protein or surface expression of TRPV4, CD36, and TLRs in macrophages. (A–D) Immunoblots of macrophage whole cell lysates following overnight treatment with 1 or 5 µM ginkgetin or vehicle. Representative immunoblots show total protein expression of CD36 (A), TRPV4 (B), TLR6 (C) and TLR4 (D) in ginkgetin-treated and untreated cells. Three independent biological replicates and 3 experimental repeats for each set of experiment were performed. (E–H) Flow cytometric analysis of macrophages treated overnight with 5 µM ginkgetin or untreated showing surface expression of TRPV4 (E), CD36 (F), TLR4 (G), and TLR6 (G) in untreated and ginkgetin-treated cells. Three independent biological replicates and 30,000 cells per condition were analyzed. (I–L) Quantitative analysis of results from (E–H). Analyzed by Two-tailed t-test with 99% confidence interval. Cells counted per experiment, 30,000; two experimental repeats.