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. 2021 Apr 14;11:8173. doi: 10.1038/s41598-021-87696-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Ginkgetin blocks oxLDL-induced upregulation of TRPV4 proteins in macrophages. Whole cell lysates were prepared from macrophages pretreated with vehicle or 10 µM ginkgetin for 3 h followed by overnight incubation with 50 µg/mL of oxLDL or nLDL. Representative immunoblots (A) show total protein expression of CD36, TRPV4, TLR2, TLR4, TLR6, and GAPDH in ginkgetin-treated and untreated cells in the presence of oxLDL or nLDL. Quantitative analysis of CD36 (B), TRPV4 (C), TLR2 (D), TLR4 (E), and TLR6 (F) proteins shown in (A). Student’s t-test, ***p < 0.001, Data represent ± SEM.