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. 2021 Apr 14;12(4):402. doi: 10.1038/s41419-021-03652-5

Fig. 3. FeTPPS attenuated caspase-11-dependent immune responses and lethality in sepsis.

Fig. 3

A–D WT or Casp11−/− mice were pretreated with FeTPPS 6 mg/kg or saline by intraperitoneal injection 1 h before intraperitoneal challenge with 25 mg/kg LPS. Serum levels of IL-1α, IL-1β, TNFα, IL-6 (A) (unpaired t-test, n = 5, ****P < 0.0001), western blots for GSDMD-NT, caspase-11 in lung (B), hematoxylin and eosin (H&E) stained sections of lung. Scale bar: 50 μm (C) and kaplan–Meier survival curves (D). Significance assessed using Log-rank test: LPS (WT n = 14) vs. LPS + FeTPPS (WT n = 14), *P = 0.0422; WT LPS (n = 14) vs. Casp11−/− LPS (n = 10), ****P < 0.0001. E–H WT or Casp11−/− mice were pretreated with FeTPPS 6 mg/kg or saline by intraperitoneal injection 1 h before subjected to cecum ligation and puncture (CLP) or sham operation. Serum levels of IL-1α, IL-1β (E), western blots for GSDMD-NT,Caspase-11 in lung (F), hematoxylin and eosin (H&E) stained sections of lung. Scale bar: 50 μm (G) and kaplan–Meier survival curves (H). Significance assessed using Log-rank test: CLP (WT n = 10) vs. CLP + FeTPPS (WT n = 10), **P = 0.0036; CLP WT (n = 10) vs. Casp11−/− (n = 10), ****P < 0.0001. I WT or Casp11−/− mice were injected with FeTPPS 6 mg/kg 1 h before intraperitoneal challenge with 25 mg/kg LPS. Plasma concentrations of PAI-1 and TAT were measured. An unpaired t-test was used in PAI-1 LPS(WT n = 5) vs. LPS+FeTPPS (WT n = 5), ****P < 0.0001; WT LPS (n = 5) vs. Casp11−/− LPS (n = 5) ***P = 0.0001 and TAT LPS (WT n = 5) vs. LPS + FeTPPS (WT n = 5), *P < 0.0299; WT LPS (n = 5) vs. Casp11−/− LPS (n = 5) ****P < 0.0001.