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. 2021 Apr 1;12:624561. doi: 10.3389/fgene.2021.624561

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Copyright © 2021 Pulczinski, Shang, Dao, Limjunyawong, Sun, Mitzner, Cheng and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Animal breeding scheme for generation of F1, F2, and F3 progenies. Nulliparous C57BL/6J female mice (F0) were sensitized to house dust mite (indicated by red box) at 8 weeks of age, and then paired with naïve C57BL/6J male mice to generate the F1 progeny. F1 males were paired with naïve C57BL/6J female mice to generate F2 progeny. F2 males were paired with naïve C57BL/6J female mice to generate F3 progeny. A subset of 6 week-males from each generation (F1, F2, and F3) were selected for adult HDM challenge followed by airway hyperresponsiveness (AHR) measurements, and gene expression analysis. Figure created with BioRender.com.