TABLE 2.
List of differential hydroxymethylated and methylated genes associated with maternal HDM exposures and increased AHR.
| Gene Symbol | Gene Name | GO function | Chromosome |
| Acox2 | Acyl-Coenzyme A oxidase 2, branched chain | Signaling receptor binding and oxidoreductase activity, acting on the CH-CH group of donors | 14 |
| Aida | Axin interactor, dorsalization associated | protein domain specific binding | 1 |
| Asmt | Acetylserotonin O-methyltransferase | Protein homodimerization activity and methyltransferase activity | X |
| Cdr1 | Cerebellar degeneration related antigen 1 | Protein binding | X |
| Col23a1 | Collagen, type XXIII, alpha 1 | Extracellular matrix structural constituent, protein binding | 11 |
| Dpcd | Deleted in primary ciliary dyskinesia | Protein binding | 19 |
| Dpy19l1 | dpy-19-like 1 | Transferase activity, transferring glycosyl groups and mannosyltransferase activity | 9 |
| Eef2 | Eukaryotic translation elongation factor 2 | Protein kinase binding | 10 |
| Erdr1 | Erythroid differentiation regulator 1 | Not available | Y |
| Foxi1 | Forkhead box I1 | DNA-binding transcription factor activity, RNA polymerase II proximal promoter sequence-specific DNA binding | 11 |
| Kcnh1 | Potassium voltage-gated channel, subfamily H (eag-related), member 1 | Signal transducer activity and ion channel activity | 1 |
| Kdm6a | Lysine (K)-specific demethylase 6A | Protein binding and dioxygenase activity | X |
| Lars2 | Leucyl-tRNA synthetase, mitochondrial | Binding and aminoacyl-tRNA editing activity | 9 |
| Luzp2 | Leucine zipper protein 2 | Extracellular region | 7 |
| Mrs2 | MRS2 magnesium transporter | Magnesium ion transmembrane transporter activity | 13 |
| Muc19 | Mucin 19 | Extracellular region, Golgi lumen, plasma membrane | 15 |
| Nox3 | NADPH oxidase 3 | Oxidoreductase activity and superoxide-generating NADPH oxidase activity | 17 |
| Nron | Non-protein coding RNA, repressor of NFAT | Not available | 2 |
| Oasl2 | 2′-5′ oligoadenylate synthetase-like 2 | RNA binding and transferase activity | 5 |
| Ranbp3l | RAN binding protein 3-like | Contributes to GTPase activator activity, Ran GTPase binding, SMAD binding | 15 |
| Rnu6 | U6 small nuclear RNA | Not available | 17 |
| Smpdl3a | Sphingomyelin phosphodiesterase, acid-like 3A | Hydrolase activity and sphingomyelin phosphodiesterase activity | 10 |
| Spag17 | Sperm associated antigen 17 | Extracellular region, cytoplasm, cytoskeleton, microtubule, cilium | 3 |
| Tmem125 | Transmembrane protein 125 | Membrane, integral component of membrane | 4 |
| Ugt8a | UDP galactosyltransferase 8A | Carbohydrate binding and glucuronosyltransferase activity | 3 |
| Xcr1 | Chemokine (C motif) receptor 1 | G protein-coupled receptor activity and chemokine receptor activity | 9 |
Hydroxymethylated and methylated DNA was captured using the hMethylCap and MethylCap kit. Significant peaks by threshold profiles at a height equivalent to an estimated false discovery rate (FDR) 0.001 were identified. Differentially methylated and hydroxymethylated regions between HDM-exposed and saline-exposed mice were annotated to their chromosomal loci and Gene Ontology (GO) categories.