TABLE 3.

mRNA levels of differential hydroxymethylated and methylated genes in F1 progenies. mRNA levels measured via RTPCR.

	A		B			C			D
	F0 Saline F1 Saline		F0 Saline F1 HDM			F0 HDM F1 Saline			F0 HDM F1 HDM
Gene	Mean	SEM	Mean	SEM	p value (B vs. A)	Mean	SEM	p value (C vs. A)	Mean	SEM	p value (D vs. C)	p value (D vs. B)
Dpy19l1	1.125	0.387	57.229	8.803	<0.001	1.205	0.339	>0.9999	41.083	8.178	<0.001	0.299
Erdr1	1.235	0.282	57.112	4.660	<0.001	0.282	0.054	1.000	15.180	2.583	0.432	<0.001
Kcnh1	0.938	0.087	263.672	24.479	<0.001	4.918	1.222	0.978	501.429	7.045	<0.001	<0.001
Lars2	0.175	0.043	32.978	7.785	0.003	0.435	0.033	>0.9999	34.603	7.404	0.004	0.998
Nron	0.455	0.388	24.942	3.301	0.042	0.297	0.143	>0.9999	48.291	3.409	<0.001	0.058
Oasl2	0.461	0.052	33.065	3.284	0.003	0.459	0.015	>0.9999	50.182	3.798	<0.001	0.249
Spag17	0.633	0.007	182.749	27.169	<0.001	2.478	0.345	0.998	323.945	42.425	<0.001	<0.001

The 2∧(−ΔΔC_t) method was used to calculate the relative expression level of transcripts normalized to Rpl19. P values derived from Ordinary One-Way ANOVA with Tukey’s test for multiple comparisons. Significant P values are in bold.