TABLE 4.

mRNA levels of epigenetic modification enzymes in F1 progenies. mRNA levels measured via RTPCR.

	A		B			C			D
	F0 Saline F1 Saline		F0 Saline F1 HDM			F0 HDM F1 Saline			F0 HDM F1 HDM
Gene	Mean	SEM	Mean	SEM	p value (B vs. A)	Mean	SEM	p value (C vs. A)	Mean	SEM	p value (D vs. C)	p value (D vs. B)
Dnmt1	1.518	0.595	0.013	0.000	0.003	0.445	0.117	0.073	0.004	0.002	0.736	>0.9999
Dnmt3a	3.671	0.503	0.182	0.023	<0.001	0.234	0.061	<0.001	0.002	0.001	0.949	0.970
Dnmt3b	2.975	0.381	0.108	0.032	<0.001	2.019	0.389	0.131	0.014	0.005	<0.001	0.996
Mecp2	2.412	0.248	0.906	0.163	0.002	1.518	0.066	0.174	0.119	0.003	0.010	0.219
Tet1	0.668	0.029	3.910	0.647	<0.001	0.825	0.165	0.983	4.450	0.184	<0.001	0.542
Tet2	1.233	0.358	1.504	0.341	0.907	1.165	0.262	0.999	1.081	0.361	0.997	0.720

The 2∧(−ΔΔC_t) method was used to calculate the relative expression level of transcripts normalized to Rpl19. P values derived from Ordinary One-Way ANOVA with Tukey’s test for multiple comparisons. Significant P values are in bold.