TABLE 5.

mRNA levels of AHR phenotypic genes in F2 and F3 progenies. mRNA levels measured via RTPCR.

F2 Generation
	A		B			C			D
	F0 Saline F1 Saline		F0 Saline F1 HDM			F0 HDM F1 Saline			F0 HDM F1 HDM
Gene	Mean	SEM	Mean	SEM	p value (B vs. A)	Mean	SEM	p value (C vs. A)	Mean	SEM	p value (D vs. C)	p value (D vs. B)
Ccnd1	1.679	0.509	14.755	2.471	0.076	1.069	0.118	0.692	17.372	5.062	0.200	0.962
Pcna	0.219	0.013	4.630	1.245	0.171	0.485	0.234	0.710	3.132	0.212	0.004	0.686
Col1a	0.749	0.121	1.336	0.204	0.230	0.138	0.008	0.090	1.427	0.080	0.009	0.972
Col3a	0.416	0.120	1.478	0.114	0.011	0.676	0.092	0.427	3.143	0.163	0.002	0.006
Muc5b	1.304	0.322	1.030	0.055	0.836	1.330	0.117	1.000	1.247	0.345	0.995	0.917
Sma	1.031	0.328	1.486	0.323	0.765	1.175	0.182	0.978	1.645	0.169	0.357	0.968
Camk2d	3.424	0.059	5.152	0.421	0.128	2.208	0.091	0.003	5.381	0.737	0.118	0.992
Mylk	1.332	0.081	1.343	0.051	1.000	0.974	0.130	0.253	1.074	0.149	0.953	0.472
F3 Generation
	A		B			C			D
	F0 Saline F1 Saline		F0 Saline F1 HDM			F0 HDM F1 Saline			F0 HDM F1 HDM
Gene	Mean	SEM	Mean	SEM	p value (B vs. A)	Mean	SEM	p value (C vs. A)	Mean	SEM	p value (D vs. C)	p value (D vs. B)
Ccnd1	5.201	0.937	32.478	7.568	0.072	3.886	0.880	0.742	29.012	3.948	0.020	0.975
Pcna	0.682	0.231	4.419	0.338	<0.001	0.871	0.135	0.892	4.092	0.646	0.041	0.967
Col1a	1.958	0.305	3.952	0.909	0.278	3.254	0.183	0.038	3.303	0.561	1.000	0.926
Col3a	0.383	0.103	1.630	0.229	0.012	0.367	0.082	0.999	1.948	0.085	<0.001	0.599
Muc5b	2.788	0.307	4.797	0.825	0.220	3.079	0.422	0.941	4.572	0.692	0.356	0.997
Sma	1.116	0.165	2.130	0.333	0.121	0.974	0.134	0.907	1.905	0.265	0.099	0.949
Camk2d	3.379	0.592	6.465	0.792	0.061	2.232	0.140	0.349	3.979	0.417	0.061	0.114
Mylk	1.410	0.091	3.461	0.586	0.078	1.509	0.080	0.844	2.934	0.308	0.049	0.855

The 2∧(−ΔΔC_t) method was used to calculate the relative expression level of transcripts normalized to Rpl19. P values derived from Ordinary One-Way ANOVA with Tukey’s test for multiple comparisons. Significant P values are in bold.