TABLE 6.
F2 | ||||||||||||
A |
B |
C |
D |
|||||||||
F0 Saline F1 Saline |
F0 Saline F1 HDM |
F0 HDM F1 Saline |
F0 HDM F1 HDM |
|||||||||
Gene | Mean | SEM | Mean | SEM | p value (B vs. A) | Mean | SEM | p value (C vs. A) | Mean | SEM | p value (D vs. C) | p value (D vs. B) |
Kcnh1 | 3.516 | 0.833 | 257.744 | 49.664 | <0.001 | 3.588 | 0.133 | >0.9999 | 374.755 | 112.999 | <0.001 | <0.001 |
Spag17 | 1.533 | 0.202 | 170.063 | 42.397 | <0.001 | 1.457 | 0.317 | >0.9999 | 121.977 | 3.923 | <0.001 | 0.058 |
F3 | ||||||||||||
A |
B |
C |
D |
|||||||||
F0 Saline F1 Saline |
F0 Saline F1 HDM |
F0 HDM F1 Saline |
F0 HDM F1 HDM |
|||||||||
Gene | Mean | SEM | Mean | SEM | p value (B vs. A) | Mean | SEM | p value (C vs. A) | Mean | SEM | p value (D vs. C) | p value (D vs. B) |
Kcnh1 | 5.106 | 0.661 | 366.325 | 51.904 | <0.001 | 2.137 | 0.598 | 0.997 | 471.418 | 77.886 | <0.001 | <0.001 |
Spag17 | 3.589 | 0.370 | 138.982 | 32.860 | <0.001 | 1.844 | 0.182 | 0.999 | 160.806 | 26.801 | <0.001 | 0.464 |
mRNA levels measured via RTPCR. The 2∧(−ΔΔCt) method was used to calculate the relative expression level of transcripts normalized to Rpl19. P values derived from Ordinary One-Way ANOVA with Tukey’s test for multiple comparisons. Significant P values are in bold.