Skip to main content
. 2021 Apr 1;12:624561. doi: 10.3389/fgene.2021.624561

TABLE 6.

mRNA levels of differential hydroxymethylated and methylated genes in F2 and F3 progenies.

F2
A
B
C
D
F0 Saline F1 Saline
F0 Saline F1 HDM
F0 HDM F1 Saline
F0 HDM F1 HDM
Gene Mean SEM Mean SEM p value (B vs. A) Mean SEM p value (C vs. A) Mean SEM p value (D vs. C) p value (D vs. B)
Kcnh1 3.516 0.833 257.744 49.664 <0.001 3.588 0.133 >0.9999 374.755 112.999 <0.001 <0.001
Spag17 1.533 0.202 170.063 42.397 <0.001 1.457 0.317 >0.9999 121.977 3.923 <0.001 0.058

F3

A
B
C
D
F0 Saline F1 Saline
F0 Saline F1 HDM
F0 HDM F1 Saline
F0 HDM F1 HDM
Gene Mean SEM Mean SEM p value (B vs. A) Mean SEM p value (C vs. A) Mean SEM p value (D vs. C) p value (D vs. B)
Kcnh1 5.106 0.661 366.325 51.904 <0.001 2.137 0.598 0.997 471.418 77.886 <0.001 <0.001
Spag17 3.589 0.370 138.982 32.860 <0.001 1.844 0.182 0.999 160.806 26.801 <0.001 0.464

mRNA levels measured via RTPCR. The 2∧(−ΔΔCt) method was used to calculate the relative expression level of transcripts normalized to Rpl19. P values derived from Ordinary One-Way ANOVA with Tukey’s test for multiple comparisons. Significant P values are in bold.